Nonenzymatically glucosylated albumin. In vitro preparation and isolation from normal human serum.

Enhanced nonenzymatic glucosylation of human serum albumin in diabetes mellitus.

Use of an ion exchange chromatographic method and a colorimetric method with thiobarbituric acid showed that levels of nonenzymatically glucosylated serum albumin were increased in patients with poorly controlled diabetes mellitus compared to controls. The two methods correlated well (r = 0.99) and clearly discriminated between normal and poorly controlled diabetic populations. The levels of gl...

SUGGESTED MECHANISM FOR THE SELECTIVE EXCRETION OF GLUCOSYLATED ALBUMIN The Effects of Diabetes Mellitus and Aging on This Process and the Origins of Diabetic Microalbuminuria BY ANJANEYULU KOWLURU,* RENU KOWLURU,*

Changes in renal function are usually found early in the course of diabetes mellitus and include microscopic albuminuria (45-200 mg protein/24 h) (1), polyuria (>4 liters/24 h), and an increase in glomerular filtration rate (GFR)' (2). These early functional changes are unaccompanied by histological changes and could well reflect the increase in plasma osmolality associated with diabetic hyperg...

Isolation and in vitro cultivation of human skin Keratinocytes and preparation of epidermal sheet

Preparation of human serum albumin microspheres (MSA) kit for lung imaging in Iran [Persian]

Radiolabeled Microspheres of human serum albumin (MSA) is of great importance in the analysis of regional lung perfusion. Preparation of MSA kit has been installed in our Division recently is carried out in two stages: 1. Production of albumin sphere particles with suitable sizes by using the spinning disk technique. 2. Formulation of MSA as a radiopharmacutical kit. So it can be labelled...

In vitro Maturation and Fertilization of Buffalo Oocytes Cultured in Media Supplemented with Bovine Serum Albumin

The aim of this study was to determine the quality of cumulus-oocyte-complexes (COCs) and the effects of bovine serum albumin (BSA) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. COCs were collected from slaughterhouse buffalo ovaries by aspiration method. Only normal grades COCs were matured for 48 hours in TCM-199 media. Two groups were created: one for the ...